abstract：BACKGROUND:Cyclosporin A (CsA) is commonly measured by immunoassay techniques that have a limited analytical range. The consequence of this is that low CsA concentrations that may be clinically significant are difficult to measure and that high concentrations require sample dilution, which introduces error and increase...

journal_title：Clinical chemistry

authors： Keevil BG,Tierney DP,Cooper DP,Morris MR

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:There have been no large-scale analyses of resource utilization comparing the overall costs to treat pediatric patients vs adult patients. Likewise, there have been no studies evaluating the costs of the various components of hospitalization (e.g., accommodation, laboratory, radiology, and drugs) among adult...

journal_title：Clinical chemistry

authors： Young DS,Sachais BS,Jefferies LC

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:Iron may promote coronary atherosclerotic disease (CAD) by increasing lipid peroxidation. Studies on biochemical or genetic markers of body iron stores as risk factors for CAD have yielded conflicting results. METHODS:We studied 849 individuals with a clear-cut definition of the CAD phenotype, i.e., with (C...

journal_title：Clinical chemistry

authors： Bozzini C,Girelli D,Tinazzi E,Olivieri O,Stranieri C,Bassi A,Trabetti E,Faccini G,Pignatti PF,Corrocher R

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:The multiple endocrine neoplasia 2 (MEN 2) syndromes [MEN 2A, MEN 2B, and familial medullary thyroid carcinoma (FMTC)] are caused by germline mutations of the RET protooncogene. Because 85% of MEN 2A patients and 30% of FMTC patients have mutations at codon 634, the recommended molecular analyses begin at ex...

journal_title：Clinical chemistry

authors： Ruiz A,Antiñolo G,Marcos I,Borrego S

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND:Proteomic technology permits the investigation of genetic metabolic diseases at the level of protein expression. Changes in the expression, polypeptide structure, and posttranslational modification of individual proteins can be detected in complex mixtures of proteins. METHODS:We used high-resolution two-di...

journal_title：Clinical chemistry

authors： Mills K,Mills PB,Clayton PT,Johnson AW,Whitehouse DB,Winchester BG

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...

journal_title：Clinical chemistry

authors： Koziol JA,Ho NJ,Felitti VJ,Beutler E

更新日期：2001-10-01 00:00:00

abstract：BACKGROUND:Thrombopoietin (TPO), the major hormone controlling platelet production, has been measured in thrombocytopenias with discordant results. The aim of our work was to assess the value of the TPO assay for differential diagnosis of thrombocytopenias in a large cohort of patients classified according to the resul...

journal_title：Clinical chemistry

authors： Gouin-Thibault I,Cassinat B,Chomienne C,Rain JD,Najean Y,Schlageter MH

更新日期：2001-09-01 00:00:00

abstract：BACKGROUND:The Elecsys beta-CrossLaps serum assay measures type I collagen degradation fragments (beta-CTx) that contain the beta-isomerized octapeptide EKAHD-beta-GGR. We investigated the analytical performance of the assay and changes in beta-CrossLaps in patients with metabolic bone diseases. METHODS:The electroche...

journal_title：Clinical chemistry

authors： Okabe R,Nakatsuka K,Inaba M,Miki T,Naka H,Masaki H,Moriguchi A,Nishizawa Y

更新日期：2001-08-01 00:00:00

abstract：BACKGROUND:A substantial proportion of the variability in the absorption and clearance of cyclosporin A (CsA) after oral administration has been attributed to variability in liver cytochrome P-450 3A4 (CYP3A4) activity and intestinal P-glycoprotein (P-gp) concentration. A polymorphism in the CYP3A4 promoter region, ter...

journal_title：Clinical chemistry

authors： von Ahsen N,Richter M,Grupp C,Ringe B,Oellerich M,Armstrong VW

更新日期：2001-06-01 00:00:00

abstract：BACKGROUND:The short half-life of the alpha-class glutathione S-transferases (GSTAs) in plasma combined with their even distribution throughout the liver lobule suggests that they may be useful complements to the more traditionally used liver markers. However, the currently available assays for measuring GSTAs in biolo...

journal_title：Clinical chemistry

authors： Dajani LK,Paus E,Warren DJ

更新日期：2001-05-01 00:00:00

abstract：BACKGROUND:Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. Although in use for almost 30 years, the clinical value of CEA in colorectal cancer is still not clear. METHODS:The literatu...

journal_title：Clinical chemistry

authors： Duffy MJ

更新日期：2001-04-01 00:00:00

abstract：BACKGROUND:Biochemical markers of bone turnover can provide prognostic information about the risk of osteoporotic fracture and are useful tools for monitoring efficacy of antiresorptive therapy. A serum-based automated assay may be of better clinical value than urinary markers because of lower imprecision and day-to-da...

journal_title：Clinical chemistry

authors： Garnero P,Borel O,Delmas PD

更新日期：2001-04-01 00:00:00

abstract：BACKGROUND:Increased plasma troponin T (cTnT), but not troponin I (cTnI), is frequently observed in end-stage renal failure patients. Although generally considered spurious, we previously reported an associated increased mortality at 12 months. METHODS:We studied long-term outcomes in 244 patients on chronic hemodialy...

journal_title：Clinical chemistry

authors： Ooi DS,Zimmerman D,Graham J,Wells GA

更新日期：2001-03-01 00:00:00

abstract：BACKGROUND:Dopa-responsive dystonia (DRD) and tetrahydrobiopterin (BH4) defects are inherited disorders characterized by monoamine neurotransmitter deficiency with decreased activity of one of the BH4-metabolizing enzymes. The aim of the study was to determine the utility of cultured skin fibroblasts for the diagnosis ...

journal_title：Clinical chemistry

authors： Bonafé L,Thöny B,Leimbacher W,Kierat L,Blau N

更新日期：2001-03-01 00:00:00

abstract：BACKGROUND:The thiopurine S:-methyltransferase (TPMT) genetic polymorphism has a significant clinical impact on the toxicity of thiopurine drugs, which are used in the treatment of leukemia and as immunosuppressants. To date, 10 mutant alleles are known that are associated with intermediate or low TPMT activity. To fac...

journal_title：Clinical chemistry

authors： Schaeffeler E,Lang T,Zanger UM,Eichelbaum M,Schwab M

更新日期：2001-03-01 00:00:00

abstract：BACKGROUND:We previously reported a family in which the propositus and both her father and paternal grandmother had high serum aminopeptidase N (CD13; EC 3.4.11.2) activity (autosomal dominant). The molecular mass of the serum CD13 polypeptide of the propositus was larger than that of normal CD13, suggesting either a m...

journal_title：Clinical chemistry

authors： Kawai M,Hara Y,Miyazato I,Hosaki S

更新日期：2001-02-01 00:00:00

abstract：BACKGROUND:Patients with trophoblastic diseases produce ordinary and irregular forms of human chorionic gonadotropin (hCG; e.g., nicked hCG, hCG missing the beta-subunit C-terminal segment, hyperglycosylated hCG, and free beta subunit) that are recognized to differing extents by automated immunometric hCG (or hCG beta)...

journal_title：Clinical chemistry

authors： Cole LA,Shahabi S,Butler SA,Mitchell H,Newlands ES,Behrman HR,Verrill HL

更新日期：2001-02-01 00:00:00

abstract：BACKGROUND:The bleeding time (BT) test predicts a higher bleeding complication rate in populations at risk for inherited or acquired platelet dysfunction, but it is of limited assistance in evaluating individual patients. There are no reports of clinical outcomes after discontinuation of the BT test. METHODS:Interview...

journal_title：Clinical chemistry

authors： Lehman CM,Blaylock RC,Alexander DP,Rodgers GM

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Near-infrared spectroscopy is proposed as a method for providing real-time urea concentrations during hemodialysis treatments. The feasibility of such noninvasive urea measurements is evaluated in undiluted dialysate fluid. METHODS:Near-infrared spectra were collected from calibration solutions of urea prep...

journal_title：Clinical chemistry

authors： Eddy CV,Arnold MA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Tartrate-resistant acid phosphatase (AcP) 5b is a marker of osteoclastic activity and bone resorption. Immunoassays for serum TRAcP may lack sensitivity and specificity because of the presence of non-bone isoform 5a. The purpose of this study was to isolate the serum isoforms, quantify their disease-related ...

journal_title：Clinical chemistry

authors： Janckila AJ,Takahashi K,Sun SZ,Yam LT

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. APPROACH:Published data from the last 3 years demonstrating the feasibi...

journal_title：Clinical chemistry

authors： Lo YM

更新日期：2000-12-01 00:00:00

abstract：BACKGROUND:The analysis of red cell folate (RCF) depends on complete hemolysis of erythrocytes, and it is assumed that complete hemolysis is achieved by 10-fold dilution of whole blood with hypotonic solutions of 10 g/L ascorbic acid/ascorbate. This report challenges this assumption. METHODS:The conventional method of...

journal_title：Clinical chemistry

authors： Wright AJ,Finglas PM,Southon S

更新日期：2000-12-01 00:00:00

abstract：PURPOSE:To review information on the use of laboratory tests in screening, diagnosis, and monitoring of acute and chronic hepatic injury. DATA SOURCES AND STUDY SELECTION:A MEDLINE search was performed for key words related to hepatic diseases, including acute hepatitis, chronic hepatitis, alcoholic hepatitis, cirrhos...

journal_title：Clinical chemistry

authors： Dufour DR,Lott JA,Nolte FS,Gretch DR,Koff RS,Seeff LB

更新日期：2000-12-01 00:00:00

abstract：BACKGROUND:Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to ca...

journal_title：Clinical chemistry

authors： Härmä H,Tarkkinen P,Soukka T,Lövgren T

更新日期：2000-11-01 00:00:00

abstract：BACKGROUND:An accurate determination of the major HFE mutation (C282Y), which is associated with hereditary hemochromatosis, is important in diagnosis and risk assessment for this disease. We report a single-tube high-throughput PCR method for the detection of C282Y. METHODS:We combined three previously described prin...

journal_title：Clinical chemistry

authors： Donohoe GG,Laaksonen M,Pulkki K,Rönnemaa T,Kairisto V

更新日期：2000-10-01 00:00:00

abstract：BACKGROUND:Human iron status is influenced by environmental and genetic factors. We hypothesized that the genetic polymorphism of haptoglobin (Hp), a hemoglobin-binding plasma protein, could affect iron status. METHODS:Reference values of serum iron status markers were compared according to Hp phenotypes (Hp 1-1, Hp 2...

journal_title：Clinical chemistry

authors： Langlois MR,Martin ME,Boelaert JR,Beaumont C,Taes YE,De Buyzere ML,Bernard DR,Neels HM,Delanghe JR

更新日期：2000-10-01 00:00:00

abstract：BACKGROUND:In recent years, there have been significant advances in the development of enzyme replacement and other therapies for lysosomal storage disorders (LSDs). Early diagnosis, before the onset of irreversible pathology, has been demonstrated to be critical for maximum efficacy of current and proposed therapies. ...

journal_title：Clinical chemistry

authors： Umapathysivam K,Whittle AM,Ranieri E,Bindloss C,Ravenscroft EM,van Diggelen OP,Hopwood JJ,Meikle PJ

更新日期：2000-09-01 00:00:00

abstract：BACKGROUND:It has been shown that a high percentage of interleukin-8 (IL-8) in blood is cell associated. Recently, a simple method for determination of cell-associated IL-8 in whole blood after cell lysis has been described. The purpose of this study was to evaluate this method, to examine the influence of preanalytic ...

journal_title：Clinical chemistry

authors： Reinsberg J,Dembinski J,Dorn C,Behrendt D,Bartmann P,van Der Ven H

更新日期：2000-09-01 00:00:00

abstract：BACKGROUND:Functional antibody surfaces were prepared on ultraflat polystyrene surfaces by physical adsorption, and the uniform distribution of monoclonal antibodies against hepatitis B surface antigen (anti-HBs) on such surfaces and the presence of dense hepatitis B surface antigen (HBsAg) particles captured by immobi...

journal_title：Clinical chemistry

authors： Qian W,Yao D,Yu F,Xu B,Zhou R,Bao X,Lu Z

更新日期：2000-09-01 00:00:00

abstract：:It is time-consuming to process and compare the clinical and marker information registered during monitoring of breast cancer patients. To facilitate the assessment, we developed a computer program for interpreting consecutive measurements. The intraindividual biological variation, the analytical precision profile, th...

journal_title：Clinical chemistry

authors： Sölétormos G,Schiøler V

更新日期：2000-08-01 00:00:00

abstract：:Hemostasis is initiated by injury to the vascular wall, leading to the deposition of platelets adhering to components of the subendothelium. Platelet adhesion requires the presence of von Willebrand factor and platelet receptors (IIb/IIIa and Ib/IX). Additional platelets are recruited to the site of injury by release ...

journal_title：Clinical chemistry

authors： Triplett DA

更新日期：2000-08-01 00:00:00

abstract：BACKGROUND:Prostate-specific antigen (PSA) is a valuable prostatic cancer biomarker that is now widely used for population screening, diagnosis, and monitoring of patients with prostate cancer. Despite the voluminous literature on this biomarker, relatively few reports have addressed the issue of its physiological func...

journal_title：Clinical chemistry

authors： Diamandis EP

更新日期：2000-07-01 00:00:00

abstract：BACKGROUND:To determine the contribution of laboratory costs to the overall costs of managing hospital patients with different diseases, we studied the costs of laboratory testing overall and in relation to the other costs incurred during hospitalization. METHODS:We used a database developed by the University HealthSy...

journal_title：Clinical chemistry

authors： Young DS,Sachais BS,Jefferies LC

更新日期：2000-07-01 00:00:00

abstract：:The historical development of a charitable children's hospital and the evolution of its clinical laboratory are presented. With the appearance of practical quantitative blood chemistry tests in the period between the two World Wars, applications to pediatrics were hampered by the need for ultramicro procedures then un...

journal_title：Clinical chemistry

authors： Meites S

更新日期：2000-07-01 00:00:00

abstract：BACKGROUND:Heparinized plasma samples allow more rapid analysis than serum samples, but preliminary studies showed lower cardiac troponin T (cTnT) results in plasma. We undertook a multicenter study to characterize this effect for cTnT and cardiac troponin I (cTnI). METHODS:Blood samples were collected with and withou...

journal_title：Clinical chemistry

authors： Gerhardt W,Nordin G,Herbert AK,Burzell BL,Isaksson A,Gustavsson E,Haglund S,Müller-Bardorff M,Katus HA

更新日期：2000-06-01 00:00:00

abstract：BACKGROUND:A single nonfunctional chimeric gene with its 5' and 3' ends corresponding to CYP21P and CYP21, respectively, is caused by unequal gene crossover in the CYP21 genes during meiosis. The presence of the chimeric CYP21P/CYP21 molecule can not be detected by conventional PCR methods and therefore may be lost in ...

journal_title：Clinical chemistry

authors： Lee HH,Chang JG,Tsai CH,Tsai FJ,Chao HT,Chung B

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:PS-341, a selective inhibitor of the proteasome, currently is under evaluation as an anticancer agent in multiple phase I clinical trials. In animal-model studies, PS-341 was rapidly removed from the vascular compartment and distributed widely, quickly approaching the limits of detection. An accurate pharmac...

journal_title：Clinical chemistry

authors： Lightcap ES,McCormack TA,Pien CS,Chau V,Adams J,Elliott PJ

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:Band 5 tartrate-resistant acid phosphatase (TrACP; EC 3. 1.3.2) consists of two isoenzymes, bands 5a and 5b, of which band 5b TrACP is considered to be derived from bone. However, no kinetic method for the specific measurement of band 5b TrACP in serum is available. Our aim was to develop a kinetic assay met...

journal_title：Clinical chemistry

authors： Nakanishi M,Yoh K,Miura T,Ohasi T,Rai SK,Uchida K

更新日期：2000-04-01 00:00:00

abstract：BACKGROUND:Concentrations of physiological response variables fluctuate over time. The present study describes within-day and seasonal fluctuations for total cholesterol, dehydroepiandrosterone sulfate (DHEA-S), hemoglobin A(1c) (HbA(1c)), IgA, prolactin, and free testosterone in blood, and estimates within- (CV(i)) an...

journal_title：Clinical chemistry

authors： Garde AH,Hansen AM,Skovgaard LT,Christensen JM

更新日期：2000-04-01 00:00:00

abstract：BACKGROUND:Spurious increases in serum troponins, especially troponin T, have been reported in patients with and without acute myocardial syndromes. METHODS:We studied 78 autopsied patients without clinical myocardial infarction (MI) and correlated histologic cardiac findings with antemortem serum creatine kinase (CK)...

journal_title：Clinical chemistry

authors： Ooi DS,Isotalo PA,Veinot JP

更新日期：2000-03-01 00:00:00